Ontology highlight
ABSTRACT:
SUBMITTER: Xu M
PROVIDER: S-EPMC5988312 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Xu Muyu M Moresco James J JJ Chang Max M Mukim Amey A Smith Davey D Diedrich Jolene K JK Yates John R JR Jones Katherine A KA
PLoS pathogens 20180523 5
HIV-1 Tat is a key regulator of viral transcription, however little is known about the mechanisms that control its turnover in T cells. Here we use a novel proteomics technique, called DiffPOP, to identify the molecular target of JIB-04, a small molecule compound that potently and selectively blocks HIV-1 Tat expression, transactivation, and virus replication in T cell lines. Mass-spectrometry analysis of whole-cell extracts from 2D10 Jurkat T cells revealed that JIB-04 targets Serine Hydroxymet ...[more]