Ontology highlight
ABSTRACT:
SUBMITTER: Cooper EM
PROVIDER: S-EPMC2666030 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Cooper Eric M EM Cutcliffe Colleen C Kristiansen Troels Z TZ Pandey Akhilesh A Pickart Cecile M CM Cohen Robert E RE
The EMBO journal 20090212 6
An unusual deubiquitinating (DUB) activity exists in HeLa cell extracts that is highly specific for cleaving K63-linked but not K48-linked polyubiquitin chains. The activity is insensitive to both N-ethyl-maleimide and ubiquitin aldehyde, indicating that it lacks an active site cysteine residue, and gel filtration experiments show that it resides in a high molecular weight (approximately 600 kDa) complex. Using a biochemical approach, we found that the K63-specific DUB activity co-fractionated t ...[more]