Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome.
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ABSTRACT:
SUBMITTER: Nonnis S
PROVIDER: S-EPMC5988613 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Publications
Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome.
Nonnis Simona S Maffioli Elisa E Zanotti Lucia L Santagata Fabiana F Negri Armando A Viola Antonella A Elliman Stephen S Tedeschi Gabriella G
EuPA open proteomics 20160107
The analysis of mesenchymal stromal cells secretome is fundamental to identify key players of processes involving these cells. Truly secreted proteins may be difficult to detect in MS based analysis of conditioned media (CM) due to proteins supplemented with fetal bovine serum (FBS). We compared different growth conditions to determine the effect of varying FBS concentration on the number and quantity of truly secreted human proteins <i>vs</i> contaminating bovine proteins. The results suggest t ...[more]
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