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Immobilization of catalase onto chitosan and chitosan-bentonite complex: A comparative study.

ABSTRACT: The immobilization of catalase onto chitosan and chitosan-bentonite was investigated and immobilization yield of 95.91 and 95.26 was obtained respectively. The optimum pH and temperature were found as 7.5 and 8.0 at 40?°C for free and immobilized enzyme. The value of Vmax decreased by 33,000-26,300, 24,500??mol (min?mg protein)-1 and Km increased by 12.5-25 and 20?mM for free and immobilized on chitosan and chitosan-bentonite respectively. The thermal stability, half life, FTIR analyses of the beads was also performed in order to characterise the structural differences. The remaining immobilized catalase onto chitosan and chitosan-bentonite activity was 50% and 70% after 20 cycles respectively. The storage stability were found as 22%, 60%, and 70% from its original activity in case of free enzyme and immobilization of chitosan, chitosan-bentonite beads respectively after 60 days.

SUBMITTER: Kaushal J

PROVIDER: S-EPMC5989589 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Immobilization of catalase onto chitosan and chitosan-bentonite complex: A comparative study.

Kaushal Jyoti J Seema Singh Gursharan G Arya Shailendra Kumar SK

Biotechnology reports (Amsterdam, Netherlands) 20180519

The immobilization of catalase onto chitosan and chitosan-bentonite was investigated and immobilization yield of 95.91 and 95.26 was obtained respectively. The optimum pH and temperature were found as 7.5 and 8.0 at 40 °C for free and immobilized enzyme. The value of Vmax decreased by 33,000-26,300, 24,500 μmol (min mg protein)-1 and Km increased by 12.5-25 and 20 mM for free and immobilized on chitosan and chitosan-bentonite respectively. The thermal stability, ...[more]

PMID: 29876307

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