Ontology highlight
ABSTRACT:
SUBMITTER: Martin NP
PROVIDER: S-EPMC5990369 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Martin Negin P NP Myers Page P Goulding Eugenia E Chen Shih-Heng SH Walker Mitzie M Porter Thomas M TM Van Gorder Lucas L Mathew Amanda A Gruzdev Artiom A Romeo Charles C
Transgenic research 20180213 1
Lentiviruses are highly efficient vehicles for delivering genes into cells. They readily transduce primary and immortalized cells in vivo and in vitro. Genes delivered by lentiviruses are incorporated and replicated as part of their host genome and therefore offer a powerful tool for creation of stable cell lines and transgenic animals. However, the zona pellucida surrounding the fertilized eggs acts as a barrier and hinders lentiviral transduction of embryos. Here, we utilize a laser, typically ...[more]