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Invasive intraductal papillary carcinoma of the bile duct masquerading as a common hepatic duct stone.

ABSTRACT:

SUBMITTER: Smith ZL

PROVIDER: S-EPMC5990414 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Invasive intraductal papillary carcinoma of the bile duct masquerading as a common hepatic duct stone.

Smith Zachary L ZL Daniel Kimberly E KE Pant Mamta M Dua Kulwinder S KS Aburajab Murad M

VideoGIE : an official video journal of the American Society for Gastrointestinal Endoscopy 20160925 4

PMID: 29905233

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Project description:IntroductionCommon bile duct (CBD) stones are a health concern for 10-20% of individuals with symptomatic gallstones, leading to health complications and placing a burden on healthcare systems. This study was initiated to investigate the changes in microbiome compositions and the metabolic signature associated with CBD stones. The research approach integrated taxonomic and functional data with metabolomics data, complemented by in vivo experiments.MethodsIn a single tertiary healthcare institution, a total of 25 patients were enrolled who had undergone endoscopic retrograde cholangiopancreatography (ERCP) between February 2019 and January 2021. We harvested DNA from bile samples acquired from these individuals. The amplification of the bacterial 16S rRNA gene V3-V4 region was conducted through polymerase chain reaction (PCR), followed by sequencing. We utilized QIIME2 for a comprehensive data analysis. Furthermore, we performed a metabolomic analysis of the bile samples using nuclear magnetic resonance (NMR) spectroscopy. For the assessment of functional gene enrichment, we employed MetaboAnalyst 5.0. Lastly, we executed in vivo experiments on C57BL/6 mice and undertook histological examinations of tissue samples.ResultsOut of the 25 study subjects, 17 underwent ERCP due to CBD stones (the CBD stone group), while the remaining 8 had the procedure for different reasons (the non-CBD stone group). An alpha diversity analysis showed a significantly greater microbial diversity in the bile samples of the non-CBD stone group (p < 0.01), and a beta diversity analysis confirmed the greater microbial compositional abundance in the gut microbiomes in this group (p = 0.01). A taxonomic analysis revealed that the abundances of Enterococcaceae and Enterococcus were higher in the bile microbiomes of the CBD stone group. A metabolic profile analysis showed that the acetate, formate, and asparagine levels were higher in the CBD stone group. A pathway enrichment analysis showed the metabolic pathways (Arginine and Proline Metabolism, Aspartate Metabolism, Glycine, and Serine Metabolism, and Ammonia Recycling pathways) that were associated with these differences. Preclinical experiments demonstrated systemic inflammation and extracellular trap formation in the CBD stone group.ConclusionsOur study highlights the importance of biliary dysbiosis and bile metabolites, specifically acetate and formate, in CBD stone development and progression. These findings have implications for the development of diagnostic and therapeutic strategies using microbiomes for CBD stones.

Dataset Information

Invasive intraductal papillary carcinoma of the bile duct masquerading as a common hepatic duct stone.

Publications

Invasive intraductal papillary carcinoma of the bile duct masquerading as a common hepatic duct stone.

Similar Datasets

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