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Development and characterization of 23 polymorphic microsatellite loci for Amentotaxus argotaenia (Taxaceae), a relict vulnerable species.

ABSTRACT:

Premise of the study

New microsatellite markers were developed for the vulnerable conifer species Amentotaxus argotaenia (Taxaceae) to investigate population genetic variation and the effects of environmental heterogeneity on genetic structure.

Methods and results

A total of 27 microsatellite loci were developed from A. argotaenia through a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, of which 23 were polymorphic. These markers yielded 1-13 alleles and 1.0-7.9 effective alleles per locus; levels of observed and expected heterozygosity varied from 0.000-1.000 and 0.000-0.873, respectively. In total, 18 of the markers were transferable to the related species A. yunnanensis.

Conclusions

These polymorphic markers are a valuable genetic resource for investigating population genetic variation and the potential for local adaptation in A. argotaenia.

SUBMITTER: Huang Q

PROVIDER: S-EPMC5991555 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Development and characterization of 23 polymorphic microsatellite loci for <i>Amentotaxus argotaenia</i> (Taxaceae), a relict vulnerable species.

Huang Qiqi Q Wang Zhen Z Wang Ting T Su Yingjuan Y

Applications in plant sciences 20180523 5

<h4>Premise of the study</h4>New microsatellite markers were developed for the vulnerable conifer species <i>Amentotaxus argotaenia</i> (Taxaceae) to investigate population genetic variation and the effects of environmental heterogeneity on genetic structure.<h4>Methods and results</h4>A total of 27 microsatellite loci were developed from <i>A. argotaenia</i> through a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, of which 23 were polymorphic. These markers yielded 1- ...[more]

PMID: 30131891

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