Ontology highlight
ABSTRACT:
SUBMITTER: Vo BT
PROVIDER: S-EPMC5992137 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Vo BaoHan T BT Kwon Jin Ah JA Li Chunliang C Finkelstein David D Xu Beisi B Orr Brent A BA Sherr Charles J CJ Roussel Martine F MF
Scientific reports 20180607 1
MYC-driven Group 3 (G3) medulloblastoma (MB) is the most aggressive of four molecular subgroups classified by transcriptome, genomic landscape and clinical outcomes. Mouse models that recapitulate human G3 MB all rely on retroviral vector-induced Myc expression driven by viral regulatory elements (Retro-Myc tumors). We used nuclease-deficient CRISPR/dCas9-based gene activation with combinatorial single guide RNAs (sgRNAs) to enforce transcription of endogenous Myc in Trp53-null neurospheres that ...[more]