Dataset Information

Decreased macrophage inflammatory protein (MIP)-1? and MIP-1? increase the risk of developing nasopharyngeal carcinoma.

ABSTRACT: BACKGROUND:The association of circulating inflammation markers with nasopharyngeal carcinoma (NPC) is still largely unclear. This study aimed to comprehensively explore the relationship between circulating cytokine levels and the subsequent risk of NPC with a two-stage epidemiologic study in southern China. METHODS:The serum levels of 33 inflammatory cytokines were first measured in a hospital-based case-control study (150 NPC patients and 150 controls) using multiplex assay platforms. Marker levels were categorized into two or more groups based on the proportion of sample measurements that was above the lower limit of detection. Odds ratios (ORs) and 95% confidence intervals (CIs) relating the serum marker concentration to the risk of NPC were computed by multivariable logistic regression models. The associations were validated in 60 patients with NPC and 120 controls in a subsequent nested case-control study within a NPC screening trial. Potential interactions between serum cytokines and Epstein-Barr virus (EBV) relating to the risk of NPC were assessed using a likelihood ratio test. RESULTS:The levels of serum macrophage inflammatory protein (MIP)-1? and MIP-1? in the highest categories were associated with a decreased risk of NPC in both the case-control study (MIP-1?: OR?=?0.49, 95% CI?=?0.26-0.95; MIP-1?: OR?=?0.47, 95% CI?=?0.22-1.00) and the nested case-control study (MIP-1?: OR?=?0.13, 95% CI?=?0.03-0.62; MIP-1?: OR?=?0.20, 95% CI?=?0.04-0.94), compared with those in the lowest categories. Furthermore, individuals with lower levels of these two cytokine markers who were EBV seropositive presented with a largely higher risk of NPC compared with patients with higher levels who were EBV seronegative in both the case-control study (MIP-1?: OR?=?16.28, 95% CI?=?7.11-37.23; MIP-1?: OR?=?12.86, 95% CI?=?5.9-28.05) and the nested case-control study (MIP-1?: OR?=?86.12, 95% CI?=?10.58-701.03; MIP-1?: OR?=?115.44, 95% CI?=?13.92-957.73). CONCLUSIONS:Decreased preclinical MIP-1? and MIP-1? levels might be associated with a subsequently increased risk of NPC. More mechanistic studies are required to fully understand this finding.

SUBMITTER: Yang MJ

PROVIDER: S-EPMC5993139 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Decreased macrophage inflammatory protein (MIP)-1α and MIP-1β increase the risk of developing nasopharyngeal carcinoma.

Yang Meng-Jie MJ Guo Jie J Ye Yan-Fang YF Chen Sui-Hong SH Peng Li-Xia LX Lin Chu-Yang CY Hu Ting T Xie Shang-Hang SH Xie Chuan-Bo CB Huang Qi-Hong QH Lu Yu-Qiang YQ Liu Qing Q Qian Chao-Nan CN Cao Su-Mei SM

Cancer communications (London, England) 20180403 1

<h4>Background</h4>The association of circulating inflammation markers with nasopharyngeal carcinoma (NPC) is still largely unclear. This study aimed to comprehensively explore the relationship between circulating cytokine levels and the subsequent risk of NPC with a two-stage epidemiologic study in southern China.<h4>Methods</h4>The serum levels of 33 inflammatory cytokines were first measured in a hospital-based case-control study (150 NPC patients and 150 controls) using multiplex assay platf ...[more]

PMID: 29764502

Similar Datasets

Project description:BackgroundAccumulating evidence indicates that dysregulation of miR-182-5p can serve as diagnostic and prognostic biomarkers for some cancers, whereas the role of miR-182-5p has not been explored in nasopharyngeal carcinoma (NPC). Our study aims to elucidate the biological function of miR-182-5p in NPC and the potential molecular mechanism involved.MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine miR-182-5p expression in NPC primary tissues and cell lines. Immunohistochemistry (IHC) for ZFP36L1 was conducted in NPC samples. Western blot was used to evaluate protein expression in cell lines. A series of functional assays were carried out to evaluate the roles of miR-182-5p and ZFP36L1 in tumor development and progression of NPC. Bioinformatics tools and luciferase reporter assays were utilized to identify the potential mechanisms of action. Moreover, rescue experiments were applied to explore whether ZFP36L1 mediated the effects of miR-182-5p in NPC.ResultsUp-regulation of miR-182-5p was significantly associated with tumor development and poor prognosis in patients with NPC. Functional study demonstrated that miR-182-5p overexpression enhanced, whereas suppression of miR-182-5p impeded NPC cell proliferation, migration, tumorigenesis and metastasis. Mechanistically, miR-182-5p interacted with ZFP36L1 at two sites in its 3' un-translated region (UTR) and repressed ZFP36L1 expression in NPC. Consistently, an inverse correlation was observed between the expression levels of miR-182-5p and ZFP36L1 using clinical NPC tissues, and down-regulation of ZFP36L1 in NPC predicts poor survival. Furthermore, overexpression of miR-182-5p in NPC was partly attributable to the transcriptional activation effect induced by hypoxia-inducible factor 1α (HIF-1α).ConclusionsOur data suggests that miR-182-5p facilitates cell proliferation and migration in NPC through its ability to down-regulate ZFP36L1 expression, and that the HIF-1α/miR-182-5p/ZFP36L1 axis may serve as a novel therapeutic target in the management of NPC.

Dataset Information

Decreased macrophage inflammatory protein (MIP)-1? and MIP-1? increase the risk of developing nasopharyngeal carcinoma.

Publications

Decreased macrophage inflammatory protein (MIP)-1α and MIP-1β increase the risk of developing nasopharyngeal carcinoma.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets