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OpenHiCAMM: High-Content Screening Software for Complex Microscope Imaging Workflows.


ABSTRACT: High-content image acquisition is generally limited to cells grown in culture, requiring complex hardware and preset imaging modalities. Here we report an open source software package, OpenHiCAMM (Open Hi Content Acquisition for μManager), that provides a flexible framework for integration of generic microscope-associated robotics and image processing with sequential work-flows. As an example, we imaged Drosophila embryos, detecting the embryos at low resolution, followed by re-imaging the detected embryos at high resolution, suitable for computational analysis and screening. The OpenHiCAMM package is easy to use and adapt for automating complex microscope image tasks. It expands our abilities for high-throughput image-based screens to a new range of biological samples, such as organoids, and will provide a foundation for bioimaging systems biology.

SUBMITTER: Booth BW 

PROVIDER: S-EPMC5993205 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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OpenHiCAMM: High-Content Screening Software for Complex Microscope Imaging Workflows.

Booth Benjamin W BW   McParland Charles C   Beattie Keith K   Fisher William W WW   Hammonds Ann S AS   Celniker Susan E SE   Frise Erwin E  

iScience 20180401


High-content image acquisition is generally limited to cells grown in culture, requiring complex hardware and preset imaging modalities. Here we report an open source software package, OpenHiCAMM (Open Hi Content Acquisition for μManager), that provides a flexible framework for integration of generic microscope-associated robotics and image processing with sequential work-flows. As an example, we imaged <i>Drosophila</i> embryos, detecting the embryos at low resolution, followed by re-imaging th  ...[more]

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