Ontology highlight
ABSTRACT:
SUBMITTER: Kiskinis E
PROVIDER: S-EPMC5993648 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Kiskinis Evangelos E Kralj Joel M JM Zou Peng P Weinstein Eli N EN Zhang Hongkang H Tsioras Konstantinos K Wiskow Ole O Ortega J Alberto JA Eggan Kevin K Cohen Adam E AE
Stem cell reports 20180517 6
Human induced pluripotent stem cell (iPSC)-derived neurons are an attractive substrate for modeling disease, yet the heterogeneity of these cultures presents a challenge for functional characterization by manual patch-clamp electrophysiology. Here, we describe an optimized all-optical electrophysiology, "Optopatch," pipeline for high-throughput functional characterization of human iPSC-derived neuronal cultures. We demonstrate the method in a human iPSC-derived motor neuron (iPSC-MN) model of am ...[more]