Ontology highlight
ABSTRACT:
SUBMITTER: Liu D
PROVIDER: S-EPMC6003224 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Liu Dongmei D Li Xia X Shen David D Novick Peter P
Molecular biology of the cell 20180117 6
The exocyst is an octameric complex that tethers secretory vesicles to the plasma membrane in preparation for fusion. We anchored each subunit with a transmembrane (TM) domain at its N- or C-terminus. Only N-terminally anchored TM-Sec3p and C-terminally anchored Exo70p-TM proved functional. These findings orient the complex with respect to the membrane and establish that Sec3p and Exo70p can function exclusively on the membrane. The functions of TM-Sec3p and Exo70p-TM were largely unaffected by ...[more]