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Interaction between the Caenorhabditis elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly.


ABSTRACT: Centrioles are microtubule-based organelles that organize the microtubule network and seed the formation of cilia and flagella. New centrioles assemble through a stepwise process dependent notably on the centriolar protein SAS-5 in Caenorhabditis elegans SAS-5 and its functional homologues in other species form oligomers that bind the centriolar proteins SAS-6 and SAS-4, thereby forming an evolutionarily conserved structural core at the onset of organelle assembly. Here, we report a novel interaction of SAS-5 with microtubules. Microtubule binding requires SAS-5 oligomerization and a disordered protein segment that overlaps with the SAS-4 binding site. Combined in vitro and in vivo analysis of select mutants reveals that the SAS-5-microtubule interaction facilitates centriole assembly in C. elegans embryos. Our findings lead us to propose that the interdependence of SAS-5 oligomerization and microtubule binding reflects an avidity mechanism, which also strengthens SAS-5 associations with other centriole components and, thus, promotes organelle assembly.

SUBMITTER: Bianchi S 

PROVIDER: S-EPMC6003225 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Interaction between the <i>Caenorhabditis elegans</i> centriolar protein SAS-5 and microtubules facilitates organelle assembly.

Bianchi Sarah S   Rogala Kacper B KB   Dynes Nicola J NJ   Hilbert Manuel M   Leidel Sebastian A SA   Steinmetz Michel O MO   Gönczy Pierre P   Vakonakis Ioannis I  

Molecular biology of the cell 20180124 6


Centrioles are microtubule-based organelles that organize the microtubule network and seed the formation of cilia and flagella. New centrioles assemble through a stepwise process dependent notably on the centriolar protein SAS-5 in <i>Caenorhabditis elegans</i> SAS-5 and its functional homologues in other species form oligomers that bind the centriolar proteins SAS-6 and SAS-4, thereby forming an evolutionarily conserved structural core at the onset of organelle assembly. Here, we report a novel  ...[more]

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