Ontology highlight
ABSTRACT:
SUBMITTER: Nakane T
PROVIDER: S-EPMC6005684 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Nakane Takanori T Kimanius Dari D Lindahl Erik E Scheres Sjors Hw SH
eLife 20180601
Macromolecular complexes that exhibit continuous forms of structural flexibility pose a challenge for many existing tools in cryo-EM single-particle analysis. We describe a new tool, called multi-body refinement, which models flexible complexes as a user-defined number of rigid bodies that move independently from each other. Using separate focused refinements with iteratively improved partial signal subtraction, the new tool generates improved reconstructions for each of the defined bodies in a ...[more]