Dataset Information

Characteristics of the bacterial microbiome in association with common intestinal parasites in irritable bowel syndrome.

ABSTRACT:

Objective

A low prevalence of intestinal parasites has been identified in individuals with irritable bowel syndrome (IBS), but potential associations with alterations in the bacterial microbiome remain largely unexplored. We aimed to investigate the relationship between parasites and bacteria in individuals with IBS in order to identify potential trans-kingdom microbial characteristics.

Design

Stool samples were collected from the Danish background population classified into IBS (n?=?119), unspecific gastrointestinal (GI) symptoms (n?=?114), and asymptomatic controls (n?=?186) based on the Rome III criteria for IBS. Bacterial (16S) and eukaryotic (18S) ribosomal DNA was sequenced, and 18S data were merged with data from conventional parasite laboratory tests. The bacterial microbiome was analyzed according to symptom group and parasite colonization status.

Results

Bacterial richness and diversity were similar for IBS and controls but higher in those with unspecific GI symptoms. A higher abundance of Bacteroides and a lower abundance of Faecalibacterium were detected in individuals with IBS and unspecific GI symptoms compared with controls. Principal component analyses indicated differences in bacterial composition related to parasite colonization rather than symptom group. Parasites were detected at the lowest frequency in the IBS group (39%) and in samples dominated by Bacteroides. Higher bacterial richness and diversity were found in parasite-positive samples from controls and those with unspecific GI symptoms but not in individuals with IBS.

Conclusion

Parasite colonization, rather than bacterial composition, differed between individuals with IBS and healthy controls. Parasite colonization was associated to a rich and diverse bacterial microbiome; however, this association was altered in IBS.

SUBMITTER: Krogsgaard LR

PROVIDER: S-EPMC6006308 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

Characteristics of the bacterial microbiome in association with common intestinal parasites in irritable bowel syndrome.

Krogsgaard Laura Rindom LR Andersen Lee O 'Brien LO' Johannesen Thor Bech TB Engsbro Anne Line AL Stensvold Christen Rune CR Nielsen Henrik Vedel HV Bytzer Peter P

Clinical and translational gastroenterology 20180619 6

<h4>Objective</h4>A low prevalence of intestinal parasites has been identified in individuals with irritable bowel syndrome (IBS), but potential associations with alterations in the bacterial microbiome remain largely unexplored. We aimed to investigate the relationship between parasites and bacteria in individuals with IBS in order to identify potential trans-kingdom microbial characteristics.<h4>Design</h4>Stool samples were collected from the Danish background population classified into IBS ( ...[more]

PMID: 29915224

Similar Datasets

Project description:Irritable bowel syndrome (IBS) is a disorder with brain-gut-microbiome alterations. Gut-directed hypnotherapy (GHT) has been shown to improve quality of life and symptoms in IBS. This therapy targets psychological coping, central nervous processing and brain-gut interaction. Studies have also demonstrated effects of hypnosis on intestinal transit and the mucosal immune system. So far, no study has examined the effect of GHT on the intestinal microbiome. This study aimed at examining microbial composition, IBS symptoms, and psychological distress before and after GHT. METHODS:Fecal samples were collected from 38 IBS patients (Rome-III criteria, mean age 44 years, 27 female, 11 male, 22 diarrhea-dominant, 12 alternating-type and 4 constipation-dominant IBS) before and after 10 weekly group sessions of GHT. Assessments in psychological (perceived stress, PSQ; psychological distress, HADS-D; quality of life, visual analogue scales) and IBS symptom-related variables (IBS severity, IBS-SSS; single symptoms, visual analogue scales) were performed with validated questionnaires. Fecal samples underwent microbial 16S rRNA analyses (regions V1?2). RESULTS:Microbial alpha diversity was stable before and after GHT (chao1 2591 ± 548 vs. 2581 ± 539, p = 0.92). No significant differences were found in relative bacterial abundances but trends of reduced abundance of Lachnospiraceae 32.18 (4.14?39.89) Median (Q1?Q3) vs. 28.11 (22.85; 35.55) and Firmicutes: Bacteroidetes ratio after GHT were observable. Significant reductions in symptom severity (323 (266?371) vs. 264 (191?331), p = 0.001) and psychological distress 17.0 (12.6?21.8) vs. 12.0 (8.3?18.0), p = 0.001, and increased well-being were found after GHT. Adequate relief after therapy was reported by 32 (84%) patients. CONCLUSION:Reductions in IBS symptoms and psychological burden were observed after gut-directed hypnotherapy, but only small changes were found in intestinal microbiota composition. The findings suggest that hypnosis may act by central nervous impact and other factors largely independent from microbiota composition modulating the brain-gut axis, possibly alterations in vagus nerve functioning and microbiota metabolism.

Dataset Information

Characteristics of the bacterial microbiome in association with common intestinal parasites in irritable bowel syndrome.

Objective

Design

Results

Conclusion

Publications

Characteristics of the bacterial microbiome in association with common intestinal parasites in irritable bowel syndrome.

Similar Datasets

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