Restoration of microRNA-130b expression suppresses osteosarcoma cell malignant behavior in vitro.

ABSTRACT: The alteration of microRNA (miR)-130b expression has been associated with promoting or suppressing numerous types of human cancer. A previous study evaluated the expression level of miR-130b in osteosarcoma tissues, and subsequently investigated the effects of miR-130b on the regulation of osteosarcoma cells malignant behavior in vitro. The study revealed that miR-130b expression levels were significantly reduced in osteosarcoma tissues and cell lines, compared with in adjacent tissues or normal cell lines. The expression of miR-130b inhibited the proliferation of osteosarcoma U-2OS and Saos-2 cells and impaired their ability to migrate, invade and form colonies. Furthermore, analysis using TargetScan and a dual-luciferase reporter assay demonstrated that miR-130b directly interacted with the 3'-untranslated region of transforming growth factor ? (TGFA) and suppressed TGFA expression. TGFA and miR-130b were also inversely expressed in osteosarcoma tissues. In addition, expression of TGFA was able to alter miR-130-regulated osteosarcoma cell proliferation, migration and invasion. Thus, the present study demonstrated that miR-130b was downregulated in osteosarcoma tissues and cell lines, whereas the expression of miR-130b suppressed osteosarcoma cell malignant behavior. At the gene level, miR-130 directly targets and inhibits TGFA expression, in addition to phosphorylated protein kinase B and epidermal growth factor receptor expression levels. Further study is required to evaluate miR-130b antitumor activity in osteosarcoma.

SUBMITTER: Wu Y 

PROVIDER: S-EPMC6006480 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature