Dataset Information

An integrated -omics analysis of the epigenetic landscape of gene expression in human blood cells.

ABSTRACT:

Background

Gene expression can be influenced by DNA methylation 1) distally, at regulatory elements such as enhancers, as well as 2) proximally, at promoters. Our current understanding of the influence of distal DNA methylation changes on gene expression patterns is incomplete. Here, we characterize genome-wide methylation and expression patterns for ~ 13 k genes to explore how DNA methylation interacts with gene expression, throughout the genome.

Results

We used a linear mixed model framework to assess the correlation of DNA methylation at ~ 400 k CpGs with gene expression changes at ~ 13 k transcripts in two independent datasets from human blood cells. Among CpGs at which methylation significantly associates with transcription (eCpGs), > 50% are distal (> 50 kb) or trans (different chromosome) to the correlated gene. Many eCpG-transcript pairs are consistent between studies and ~ 90% of neighboring eCpGs associate with the same gene, within studies. We find that enhancers (P < 5e-18) and microRNA genes (P = 9e-3) are overrepresented among trans eCpGs, and insulators and long intergenic non-coding RNAs are enriched among cis and distal eCpGs. Intragenic-eCpG-transcript correlations are negative in 60-70% of occurrences and are enriched for annotated gene promoters and enhancers (P < 0.002), highlighting the importance of intragenic regulation. Gene Ontology analysis indicates that trans eCpGs are enriched for transcription factor genes and chromatin modifiers, suggesting that some trans eCpGs represent the influence of gene networks and higher-order transcriptional control.

Conclusions

This work sheds new light on the interplay between epigenetic changes and gene expression, and provides useful data for mining biologically-relevant results from epigenome-wide association studies.

SUBMITTER: Kennedy EM

PROVIDER: S-EPMC6006777 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

An integrated -omics analysis of the epigenetic landscape of gene expression in human blood cells.

Kennedy Elizabeth M EM Goehring George N GN Nichols Michael H MH Robins Chloe C Mehta Divya D Klengel Torsten T Eskin Eleazar E Smith Alicia K AK Conneely Karen N KN

BMC genomics 20180619 1

<h4>Background</h4>Gene expression can be influenced by DNA methylation 1) distally, at regulatory elements such as enhancers, as well as 2) proximally, at promoters. Our current understanding of the influence of distal DNA methylation changes on gene expression patterns is incomplete. Here, we characterize genome-wide methylation and expression patterns for ~ 13 k genes to explore how DNA methylation interacts with gene expression, throughout the genome.<h4>Results</h4>We used a linear mixed mo ...[more]

PMID: 29914364

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Dataset Information

An integrated -omics analysis of the epigenetic landscape of gene expression in human blood cells.

Background

Results

Conclusions

Publications

An integrated -omics analysis of the epigenetic landscape of gene expression in human blood cells.

Similar Datasets

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