The mitochondrial oxidoreductase CHCHD4 is present in a semi-oxidized state in vivo.
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ABSTRACT:
SUBMITTER: Erdogan AJ
PROVIDER: S-EPMC6007816 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
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The mitochondrial oxidoreductase CHCHD4 is present in a semi-oxidized state in vivo.
Erdogan Alican J AJ Ali Muna M Habich Markus M Salscheider Silja L SL Schu Laura L Petrungaro Carmelina C Thomas Luke W LW Ashcroft Margaret M Leichert Lars I LI Roma Leticia Prates LP Riemer Jan J
Redox biology 20180324
Disulfide formation in the mitochondrial intermembrane space is an essential process catalyzed by a disulfide relay machinery. In mammalian cells, the key enzyme in this machinery is the oxidoreductase CHCHD4/Mia40. Here, we determined the in vivo CHCHD4 redox state, which is the major determinant of its cellular activity. We found that under basal conditions, endogenous CHCHD4 redox state in cultured cells and mouse tissues was predominantly oxidized, however, degrees of oxidation in different ...[more]
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