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Anti-cancer binary system activated by bacteriophage HK022 integrase.

ABSTRACT: The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific hTERT promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under hTERT. In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various hTERT suppressing factors induced by the mono system.

SUBMITTER: Elias A

PROVIDER: S-EPMC6007955 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Anti-cancer binary system activated by bacteriophage HK022 integrase.

Elias Amer A Gritsenko Natasha N Gorovits Rena R Spector Itay I Prag Gali G Yagil Ezra E Kolot Mikhail M

Oncotarget 20180608 44

The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific <i>hTERT</i> promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly ex ...[more]

PMID: 29938000

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