Ontology highlight
ABSTRACT:
SUBMITTER: Woolston BM
PROVIDER: S-EPMC6008399 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Woolston Benjamin M BM King Jason R JR Reiter Michael M Van Hove Bob B Stephanopoulos Gregory G
Nature communications 20180619 1
Due to volatile sugar prices, the food vs fuel debate, and recent increases in the supply of natural gas, methanol has emerged as a promising feedstock for the bio-based economy. However, attempts to engineer Escherichia coli to metabolize methanol have achieved limited success. Here, we provide a rigorous systematic analysis of several potential pathway bottlenecks. We show that regeneration of ribulose 5-phosphate in E. coli is insufficient to sustain methanol assimilation, and overcome this b ...[more]