Unknown

Dataset Information

0

Expression of miR-652-3p and Effect on Apoptosis and Drug Sensitivity in Pediatric Acute Lymphoblastic Leukemia.


ABSTRACT: MicroRNAs (miRNAs) expression profiles were screened in plasma samples from pediatric patients with acute lymphoblastic leukemia (ALL) and healthy controls, using qRT-PCR-based TaqMan low-density miRNA arrays. MiR-652-3p (a circulating miRNA) was downregulated in new diagnosis (ND) patients compared with healthy controls. The levels of miR652-3p were restored in complete remission (CR) but were downregulated again in disease relapse (RE). The expression pattern of miR-652-3p was validated in bone marrow (BM) samples from other pediatric ALL patients. MiR-652-3p was significantly upregulated in BM when the patients (n=86) achieved CR, as compared with the matched ND samples (p<0.001). Moreover, the miR-652-3p levels in BM decreased again in two patients at RE. In addition, the lymphoblastic leukemia cell lines Reh and RS4:11 were found to have lower levels of miR-625-3p than the normal B-cell line. Overexpression of miR-652-3p using agomir increased the sensitivity to vincristine and cytarabine (all p<0.05) and promoted apoptosis (both p<0.05) in Reh and RS4:11 cells. In conclusion, the results suggested that a low level of miR-652-3p might be involved in the pathogenesis of pediatric ALL. Overexpression of miR-652-3p might suppress lymphoblastic leukemia cells, promoting apoptosis and increasing sensitivity to chemotherapeutic drugs.

SUBMITTER: Jiang Q 

PROVIDER: S-EPMC6008837 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7408026 | biostudies-literature
| S-EPMC7574699 | biostudies-literature
| S-EPMC3084917 | biostudies-literature
| S-EPMC4053464 | biostudies-literature
| S-EPMC5243866 | biostudies-literature
| S-EPMC4030177 | biostudies-literature
| S-EPMC7838722 | biostudies-literature
2011-02-11 | E-GEOD-23024 | biostudies-arrayexpress
2011-02-11 | GSE23024 | GEO
| S-EPMC3789458 | biostudies-literature