Ontology highlight
ABSTRACT:
SUBMITTER: Potapov V
PROVIDER: S-EPMC6009661 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Potapov Vladimir V Fu Xiaoqing X Dai Nan N Corrêa Ivan R IR Tanner Nathan A NA Ong Jennifer L JL
Nucleic acids research 20180601 11
Ribonucleic acid (RNA) is capable of hosting a variety of chemically diverse modifications, in both naturally-occurring post-transcriptional modifications and artificial chemical modifications used to expand the functionality of RNA. However, few studies have addressed how base modifications affect RNA polymerase and reverse transcriptase activity and fidelity. Here, we describe the fidelity of RNA synthesis and reverse transcription of modified ribonucleotides using an assay based on Pacific Bi ...[more]