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Genome-wide analysis reveals no evidence of trans chromosomal regulation of mammalian immune development.

ABSTRACT: It has been proposed that interactions between mammalian chromosomes, or transchromosomal interactions (also known as kissing chromosomes), regulate gene expression and cell fate determination. Here we aimed to identify novel transchromosomal interactions in immune cells by high-resolution genome-wide chromosome conformation capture. Although we readily identified stable interactions in cis, and also between centromeres and telomeres on different chromosomes, surprisingly we identified no gene regulatory transchromosomal interactions in either mouse or human cells, including previously described interactions. We suggest that advances in the chromosome conformation capture technique and the unbiased nature of this approach allow more reliable capture of interactions between chromosomes than previous methods. Overall our findings suggest that stable transchromosomal interactions that regulate gene expression are not present in mammalian immune cells and that lineage identity is governed by cis, not trans chromosomal interactions.

SUBMITTER: Johanson TM

PROVIDER: S-EPMC6010296 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Genome-wide analysis reveals no evidence of trans chromosomal regulation of mammalian immune development.

Johanson Timothy M TM Coughlan Hannah D HD Lun Aaron T L ATL Bediaga Naiara G NG Naselli Gaetano G Garnham Alexandra L AL Harrison Leonard C LC Smyth Gordon K GK Allan Rhys S RS

PLoS genetics 20180608 6

It has been proposed that interactions between mammalian chromosomes, or transchromosomal interactions (also known as kissing chromosomes), regulate gene expression and cell fate determination. Here we aimed to identify novel transchromosomal interactions in immune cells by high-resolution genome-wide chromosome conformation capture. Although we readily identified stable interactions in cis, and also between centromeres and telomeres on different chromosomes, surprisingly we identified no gene r ...[more]

PMID: 29883495

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Dataset Information

Genome-wide analysis reveals no evidence of trans chromosomal regulation of mammalian immune development.

Publications

Genome-wide analysis reveals no evidence of trans chromosomal regulation of mammalian immune development.

Similar Datasets

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