Enhanced plasmacytoid dendritic cell antiviral responses after omalizumab.

ABSTRACT:

Background

Atopy and viral respiratory tract infections synergistically promote asthma exacerbations. IgE cross-linking inhibits critical virus-induced IFN-α responses of plasmacytoid dendritic cells (pDCs), which can be deficient in patients with allergic asthma.

Objective

We sought to determine whether reducing IgE levels in vivo with omalizumab treatment increases pDC antiviral IFN-α responses in inner-city children with asthma.

Methods

PBMCs and pDCs isolated from children with exacerbation-prone asthma before and during omalizumab treatment were stimulated ex vivo with rhinovirus and influenza in the presence or absence of IgE cross-linking. IFN-α levels were measured in supernatants, and mRNA expression of IFN-α pathway genes was determined by using quantitative

SUBMITTER: Gill MA 

PROVIDER: S-EPMC6013066 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature