Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection.

ABSTRACT:

Introduction

Treatment of infection within bone is difficult, and conventional surgical treatment brings intense pain to the patients physically and mentally. There is an urgent need to develop injectable nano- and/or micro-medicine for minimally invasive treatment of osteomyelitis.

Methods

In this paper, amino acid (L-lysine [Lys]) was mineralized into yolk-shell structured CaCO₃ microspheres (MSs). The morphologies of the obtained MSs were investigated by scanning electron microscopy and transmission electron microscopy. The composition of CaCO₃ MSs was identified by using Fourier transform infrared spectroscopy. The as-prepared CaCO₃ MSs were examined with power X-ray diffraction analysis to obtain the crystallographic structure of the MSs.

Results

The as prepared Lys encapsulated CaCO₃ MSs (Lys@CaCO₃ MSs) were used as micro-drug to improve acidic environment of osteomyelitis caused by bacterial infection and promote osteoblast proliferation under oxidative stress. These pH responsive Lys@CaCO₃ MSs have a drug loading efficiency of 89.8 wt % and drug loading content (DLC) of 22.3 wt %.

Conclusion

Our results demonstrated that Lys@CaCO₃ MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H₂O₂ at pH = 5.5.

SUBMITTER: Chen Z 

PROVIDER: S-EPMC6014387 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature