Ontology highlight
ABSTRACT:
SUBMITTER: Yang M
PROVIDER: S-EPMC6017383 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Yang Min M Hong Haofei H Liu Shaozhong S Zhao Xinrui X Wu Zhimeng Z
Molecules (Basel, Switzerland) 20180119 1
Chitosan macro-particles prepared by the neutralization method were applied to Sortase A (SrtA) immobilization using glutaraldehyde as a crosslinking agent. The particles were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Response surface methodology (RSM) was employed to optimize the immobilization process. An average specific activity of 3142 U (mg protein)<sup>-1</sup> was obtained under optimized immobilization conditions (chitosan co ...[more]