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Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis.


ABSTRACT: (3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of (3S)-acetoin and (2S,3S)-2,3-butanediol from meso-2,3-butanediol. First, E. coli co-expressing (2R,3R)-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin was developed for (3S)-acetoin production from meso-2,3-butanediol. Maximum (3S)-acetoin concentration of 72.38 g/L with the stereoisomeric purity of 94.65% was achieved at 24 h under optimal conditions. Subsequently, we developed another biocatalyst co-expressing (2S,3S)-2,3-butanediol dehydrogenase and formate dehydrogenase for (2S,3S)-2,3-butanediol production from (3S)-acetoin. Synchronous catalysis together with two biocatalysts afforded 38.41 g/L of (2S,3S)-butanediol with stereoisomeric purity of 98.03% from 40 g/L meso-2,3-butanediol. These results exhibited the potential for (3S)-acetoin and (2S,3S)-butanediol production from meso-2,3-butanediol as a substrate via whole-cell biocatalysis.

SUBMITTER: He Y 

PROVIDER: S-EPMC6017632 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis.

He Yuanzhi Y   Chen Feixue F   Sun Meijing M   Gao Huifang H   Guo Zewang Z   Lin Hui H   Chen Jiebo J   Jin Wensong W   Yang Yunlong Y   Zhang Liaoyuan L   Yuan Jun J  

Molecules (Basel, Switzerland) 20180319 3


(3<i>S</i>)-Acetoin and (2<i>S</i>,3<i>S</i>)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of (3<i>S</i>)-acetoin and (2<i>S</i>,3<i>S</i>)-2,3-butanediol from <i>meso</i>-2,3-butanediol. First, <i>E. coli</i> co-expressing (2<i>R</i>,3<i>R</i>)-2,3-butanediol dehydr  ...[more]

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