Ontology highlight
ABSTRACT:
SUBMITTER: Miciak JJ
PROVIDER: S-EPMC6021080 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Miciak Jessica J JJ Hirshberg Jason J Bunz Fred F
PloS one 20180627 6
The adenoviruses are essential tools for basic research and therapeutic development. Robust methods for the generation of mutant and recombinant viruses are crucial for these diverse applications. Here we describe a simple plasmid-based method that permits highly efficient modification of the adenoviral genome and rapid production of high-titer virus stocks. The 36-kilobase genome of adenovirus serotype 5 was divided into seven tractable blocks that could be individually modified in a single ste ...[more]