Lipid-exchange in nanodiscs discloses membrane boundaries of cytochrome-P450 reductase.
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ABSTRACT:
SUBMITTER: Barnaba C
PROVIDER: S-EPMC6022741 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Publications
Lipid-exchange in nanodiscs discloses membrane boundaries of cytochrome-P450 reductase.
Barnaba Carlo C Ravula Thirupathi T Medina-Meza Ilce G IG Im Sang-Choul SC Anantharamaiah G M GM Waskell Lucy L Ramamoorthy Ayyalusamy A
Chemical communications (Cambridge, England) 20180601 49
Lipids are critical for the function of membrane proteins. NADPH-cytochrome-P450-reductase, the sole electron transferase for microsomal oxygenases, possesses a conformational dynamics entwined with its topology. Here, we use peptide-nanodiscs to unveil cytochrome-P450-reductase's lipid boundaries, demonstrating a protein-driven enrichment of ethanolamine lipids (by 25%) which ameliorates by 3-fold CPR's electron-transfer ability. ...[more]
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