A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures.
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ABSTRACT:
SUBMITTER: Septiadi D
PROVIDER: S-EPMC6026119 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Publications
A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures.
Septiadi D D Bourquin J J Durantie E E Petri-Fink A A Rothen-Rutishauser B B
Scientific reports 20180629 1
Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes. ...[more]
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