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Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts.


ABSTRACT: Neuronal polarity relies on the selective localization of cargo to axons or dendrites. The molecular motor kinesin-1 moves cargo into axons but is also active in dendrites. This raises the question of how kinesin-1 activity is regulated to maintain the compartment-specific localization of cargo. Our in vivo structure-function analysis of endogenous Drosophila melanogaster kinesin-1 reveals a novel role for autoinhibition in enabling the dendrite-specific localization of Golgi outposts. Mutations that disrupt kinesin-1 autoinhibition result in the axonal mislocalization of Golgi outposts. Autoinhibition also regulates kinesin-1 localization. Uninhibited kinesin-1 accumulates in axons and is depleted from dendrites, correlating with the change in outpost distribution and dendrite growth defects. Genetic interaction tests show that a balance of kinesin-1 inhibition and dynein activity is necessary to localize Golgi outposts to dendrites and keep them from entering axons. Our data indicate that kinesin-1 activity is precisely regulated by autoinhibition to achieve the selective localization of dendritic cargo.

SUBMITTER: Kelliher MT 

PROVIDER: S-EPMC6028532 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts.

Kelliher Michael T MT   Yue Yang Y   Ng Ashley A   Kamiyama Daichi D   Huang Bo B   Verhey Kristen J KJ   Wildonger Jill J  

The Journal of cell biology 20180504 7


Neuronal polarity relies on the selective localization of cargo to axons or dendrites. The molecular motor kinesin-1 moves cargo into axons but is also active in dendrites. This raises the question of how kinesin-1 activity is regulated to maintain the compartment-specific localization of cargo. Our in vivo structure-function analysis of endogenous <i>Drosophila melanogaster</i> kinesin-1 reveals a novel role for autoinhibition in enabling the dendrite-specific localization of Golgi outposts. Mu  ...[more]

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