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Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis.

ABSTRACT: The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptome analysis has not been reported. Here, we performed RNA-seq on over 4,000 individual cells from 22 brain regions of human mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and the pons showed especially high percentage of astrocytes. Embryonic neurons were not as diverse as adult neurons, although they possessed important features of their destinies in adults. Neuron development was unsynchronized in the cerebral cortex, as dorsal regions appeared to be more mature than ventral regions at this stage. Region-specific genes were comprehensively identified in each neuronal sub-cluster, and a large proportion of these genes were neural disease related. Our results present a systematic landscape of the regionalized gene expression and neuron maturation of the human cerebral cortex.

SUBMITTER: Fan X

PROVIDER: S-EPMC6028726 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis.

Fan Xiaoying X Dong Ji J Zhong Suijuan S Wei Yuan Y Wu Qian Q Yan Liying L Yong Jun J Sun Le L Wang Xiaoye X Zhao Yangyu Y Wang Wei W Yan Jie J Wang Xiaoqun X Qiao Jie J Tang Fuchou F

Cell research 20180604 7

The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptome analysis has not been reported. Here, we performed RNA-seq on over 4,000 individual cells from 22 brain regions of human mid-gestation embryos. We identified 29 cell sub-clusters, which showed different proportions in each region and the pons showed especially high percentage of astrocytes. Embryonic neur ...[more]

PMID: 29867213

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Dataset Information

Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis.

Publications

Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis.

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