Ontology highlight
ABSTRACT:
SUBMITTER: Clarke NI
PROVIDER: S-EPMC6031641 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Clarke Nicholas I NI Royle Stephen J SJ
Nature communications 20180704 1
A current challenge is to develop tags to precisely visualize proteins in cells by light and electron microscopy. Here, we introduce FerriTag, a genetically-encoded chemically-inducible tag for correlative light-electron microscopy. FerriTag is a fluorescent recombinant electron-dense ferritin particle that can be attached to a protein-of-interest using rapamycin-induced heterodimerization. We demonstrate the utility of FerriTag for correlative light-electron microscopy by labeling proteins asso ...[more]