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Optogenetic Control of Voltage-Gated Calcium Channels.

ABSTRACT: Voltage-gated Ca²⁺ (Ca_V ) channels mediate Ca²⁺ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca_V channel modulators, to enable photo-tunable modulation of Ca_V channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca_V channels could find broad applications in interrogating a wide range of Ca_V -mediated physiological processes.

SUBMITTER: Ma G

PROVIDER: S-EPMC6032918 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Optogenetic Control of Voltage-Gated Calcium Channels.

Ma Guolin G Liu Jindou J Ke Yuepeng Y Liu Xin X Li Minyong M Wang Fen F Han Gang G Huang Yun Y Wang Youjun Y Zhou Yubin Y

Angewandte Chemie (International ed. in English) 20180416 24

Voltage-gated Ca2+ (CaV ) channels mediate Ca2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded CaV channel modulators, to enable photo-tunable modulation of CaV channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for CaV channels could find broad applications ...[more]

PMID: 29569306

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