Dataset Information

CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation.

ABSTRACT: Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20?mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.

SUBMITTER: Attri P

PROVIDER: S-EPMC6033864 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation.

Attri Pankaj P Han Jeongmin J Choi Sooho S Choi Eun Ha EH Bogaerts Annemie A Lee Weontae W

Scientific reports 20180705 1

Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the stru ...[more]

PMID: 29977069

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Dataset Information

CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation.

Publications

CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation.

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