Unknown

Dataset Information

0

Huanglongmycin A-C, Cytotoxic Polyketides Biosynthesized by a Putative Type II Polyketide Synthase From Streptomyces sp. CB09001.

ABSTRACT: Three natural products of nonaketide biosynthetic origin, probably biosynthesized from nine molecules of malonyl-CoA, have been isolated. Herein we described the isolation and structure elucidation of huanglongmycin (HLM) A-C and identification of the putative hlm biosynthetic gene cluster from Streptomyces sp. CB09001, isolated from a karstic cave in Xiangxi, China. Albeit previously isolated, HLM A was reported for the first time to exhibit moderate cytotoxicity against A549 lung cancer cell line (IC50 = 13.8 ± 1.5 ?M) and weak antibacterial activity against gram-negative clinical isolates. A putative biosynthetic pathway for HLM A, featuring a nonaketide-specific type II polyketide synthase, was proposed. It would be consistent with the isolation of HLM B and C, which are two new natural products and likely shunt metabolites during HLM A biosynthesis.

SUBMITTER: Jiang L 

PROVIDER: S-EPMC6036704 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Huanglongmycin A-C, Cytotoxic Polyketides Biosynthesized by a Putative Type II Polyketide Synthase From <i>Streptomyces</i> sp. CB09001.

Jiang Lin L   Pu Hong H   Xiang Jingxi J   Su Meng M   Yan Xiaohui X   Yang Dong D   Zhu Xiangcheng X   Shen Ben B   Duan Yanwen Y   Huang Yong Y  

Frontiers in chemistry 20180626

Three natural products of nonaketide biosynthetic origin, probably biosynthesized from nine molecules of malonyl-CoA, have been isolated. Herein we described the isolation and structure elucidation of huanglongmycin (HLM) A-C and identification of the putative <i>hlm</i> biosynthetic gene cluster from <i>Streptomyces</i> sp. CB09001, isolated from a karstic cave in Xiangxi, China. Albeit previously isolated, HLM A was reported for the first time to exhibit moderate cytotoxicity against A549 lung  ...[more]

Similar Datasets

Unknown Biosynthesis of aliphatic polyketides by type III polyketide synthase and methyltransferase in Bacillus subtilis.
| S-EPMC2715739 | biostudies-literature
Unknown Putative monofunctional type I polyketide synthase units: a dinoflagellate-specific feature?
| S-EPMC3489724 | biostudies-literature
Unknown Cycloheximide and actiphenol production in Streptomyces sp. YIM56141 governed by single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase.
| S-EPMC4051428 | biostudies-literature
Unknown Identification of unique type II polyketide synthase genes in soil.
| S-EPMC1087561 | biostudies-literature
Unknown Biosynthesis of akaeolide and lorneic acids and annotation of type I polyketide synthase gene clusters in the genome of Streptomyces sp. NPS554.
| S-EPMC4306953 | biostudies-literature
Unknown Rare Streptomyces sp. polyketides as modulators of K-Ras localisation.
| S-EPMC5644991 | biostudies-literature
Unknown Isolation and sequence analysis of polyketide synthase genes from the daunomycin-producing Streptomyces sp. strain C5.
| S-EPMC196968 | biostudies-other
Unknown Structural basis for selectivity in a highly reducing type II polyketide synthase.
| S-EPMC7556716 | biostudies-literature
Unknown Involvement of AlpV, a new member of the Streptomyces antibiotic regulatory protein family, in regulation of the duplicated type II polyketide synthase alp gene cluster in Streptomyces ambofaciens.
| S-EPMC1065233 | biostudies-literature
Unknown Type II thioesterase ScoT, associated with Streptomyces coelicolor A3(2) modular polyketide synthase Cpk, hydrolyzes acyl residues and has a preference for propionate.
| S-EPMC2643599 | biostudies-literature