Dataset Information

Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.

ABSTRACT: Asthma is a chronic inflammatory disease process involving the conductive airways of the human lung. The dysregulated inflammatory response in this disease process may involve multiple cell-cell interactions mediated by signaling molecules, including lipid mediators. Extracellular vesicles (EVs) are lipid membrane particles that are now recognized as critical mediators of cell-cell communication. Here, we compared the lipid composition and presence of specific lipid mediators in airway EVs purified from the bronchoalveolar lavage (BAL) fluid of healthy controls and asthmatic subjects with and without second-hand smoke (SHS) exposure. Airway exosome concentrations were increased in asthmatics, and correlated with blood eosinophilia and serum IgE levels. Frequencies of HLA-DR⁺ and CD54⁺ exosomes were also significantly higher in asthmatics. Lipidomics analysis revealed that phosphatidylglycerol, ceramide-phosphates, and ceramides were significantly reduced in exosomes from asthmatics compared to the non-exposed control groups. Sphingomyelin 34:1 was more abundant in exosomes of SHS-exposed asthmatics compared to healthy controls. Our results suggest that chronic airway inflammation may be driven by alterations in the composition of lipid mediators within airway EVs of human subjects with asthma.

SUBMITTER: Hough KP

PROVIDER: S-EPMC6037776 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.

Hough Kenneth P KP Wilson Landon S LS Trevor Jennifer L JL Strenkowski John G JG Maina Njeri N Kim Young-Il YI Spell Marion L ML Wang Yong Y Chanda Diptiman D Dager Jose Rodriguez JR Sharma Nirmal S NS Curtiss Miranda M Antony Veena B VB Dransfield Mark T MT Chaplin David D DD Steele Chad C Barnes Stephen S Duncan Steven R SR Prasain Jeevan K JK Thannickal Victor J VJ Deshane Jessy S JS

Scientific reports 20180709 1

Asthma is a chronic inflammatory disease process involving the conductive airways of the human lung. The dysregulated inflammatory response in this disease process may involve multiple cell-cell interactions mediated by signaling molecules, including lipid mediators. Extracellular vesicles (EVs) are lipid membrane particles that are now recognized as critical mediators of cell-cell communication. Here, we compared the lipid composition and presence of specific lipid mediators in airway EVs purif ...[more]

PMID: 29985427

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Dataset Information

Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.

Publications

Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.

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