Ontology highlight
ABSTRACT:
SUBMITTER: Chozinski TJ
PROVIDER: S-EPMC6039510 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Chozinski Tyler J TJ Mao Chenyi C Halpern Aaron R AR Pippin Jeffrey W JW Shankland Stuart J SJ Alpers Charles E CE Najafian Behzad B Vaughan Joshua C JC
Scientific reports 20180710 1
Although light microscopy is a powerful tool for the assessment of kidney physiology and pathology, it has traditionally been unable to resolve structures separated by less than the ~250 nm diffraction limit of visible light. Here, we report on the optimization, validation, and application of a recently developed super-resolution fluorescence microscopy method, called expansion microscopy (ExM), for volumetric interrogation of mouse and human kidney tissue with 70-75 nm lateral and ~250 nm axial ...[more]