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Continuous volumetric imaging via an optical phase-locked ultrasound lens.

ABSTRACT: In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficking of immune cells.

SUBMITTER: Kong L

PROVIDER: S-EPMC4551496 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Continuous volumetric imaging via an optical phase-locked ultrasound lens.

Kong Lingjie L Tang Jianyong J Little Justin P JP Yu Yang Y Lämmermann Tim T Lin Charles P CP Germain Ronald N RN Cui Meng M

Nature methods 20150713 8

In vivo imaging at high spatiotemporal resolution is key to the understanding of complex biological systems. We integrated an optical phase-locked ultrasound lens into a two-photon fluorescence microscope and achieved microsecond-scale axial scanning, thus enabling volumetric imaging at tens of hertz. We applied this system to multicolor volumetric imaging of processes sensitive to motion artifacts, including calcium dynamics in behaving mouse brain and transient morphology changes and trafficki ...[more]

PMID: 26167641

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