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Biosynthesis of the thiamin-thiazole in eukaryotes: identification of a thiazole tautomer intermediate.


ABSTRACT: Thiamin thiazole biosynthesis in eukaryotes is still not completely understood. In this report, a late intermediate, tightly bound to the active site of the Saccharomyces cerevisiae thiazole synthase, was identified as an adenylated thiazole tautomer. The reactivity of this unusual compound was evaluated. Its identification provides an additional molecular snapshot of the complex reaction sequence catalyzed by the eukaryotic thiazole synthase and identifies the final step of the thiamin-thiazole biosynthesis.

SUBMITTER: Chatterjee A 

PROVIDER: S-EPMC6040654 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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Biosynthesis of the thiamin-thiazole in eukaryotes: identification of a thiazole tautomer intermediate.

Chatterjee Abhishek A   Schroeder Frank C FC   Jurgenson Christopher T CT   Ealick Steven E SE   Begley Tadhg P TP  

Journal of the American Chemical Society 20080725 34


Thiamin thiazole biosynthesis in eukaryotes is still not completely understood. In this report, a late intermediate, tightly bound to the active site of the Saccharomyces cerevisiae thiazole synthase, was identified as an adenylated thiazole tautomer. The reactivity of this unusual compound was evaluated. Its identification provides an additional molecular snapshot of the complex reaction sequence catalyzed by the eukaryotic thiazole synthase and identifies the final step of the thiamin-thiazole  ...[more]

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