Dataset Information

Vitamin D, DNA methylation, and breast cancer.

ABSTRACT:

Background

Vitamin D has anticarcinogenic and immune-related properties and may protect against some diseases, including breast cancer. Vitamin D affects gene transcription and may influence DNA methylation.

Methods

We studied the relationships between serum vitamin D, DNA methylation, and breast cancer using a case-cohort sample (1070 cases, 1277 in subcohort) of non-Hispanic white women. For our primary analysis, we used robust linear regression to examine the association between serum 25-hydroxyvitamin D (25(OH)D) and methylation within a random sample of the cohort ("subcohort"). We focused on 198 CpGs in or near seven vitamin D-related genes. For these 198 candidate CpG loci, we also examined how multiplicative interactions between methylation and 25(OH)D were associated with breast cancer risk. This was done using Cox proportional hazards models and the full case-cohort sample. We additionally conducted an exploratory epigenome-wide association study (EWAS) of the association between 25(OH)D and DNA methylation in the subcohort.

Results

Of the CpGs in vitamin D-related genes, cg21201924 (RXRA) had the lowest p value for association with 25(OH)D (p?=?0.0004). Twenty-two other candidate CpGs were associated with 25(OH)D (p?-5), indicating a larger methylation-breast cancer hazard ratio in those with high serum 25(OH)D concentrations. We also observed statistically significant (p?-8), cg06177555 (SPN; p?=?9.8?×?10^-8), and cg13243168 (SMARCD2; p?=?2.9?×?10^-7).

Conclusions

25(OH)D concentrations were associated with DNA methylation of CpGs in several vitamin D-related genes, with potential links to immune function-related genes. Methylation of CpGs in vitamin D-related genes may interact with 25(OH)D to affect the risk of breast cancer.

SUBMITTER: O'Brien KM

PROVIDER: S-EPMC6042268 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Publications

Vitamin D, DNA methylation, and breast cancer.

O'Brien Katie M KM Sandler Dale P DP Xu Zongli Z Kinyamu H Karimi HK Taylor Jack A JA Weinberg Clarice R CR

Breast cancer research : BCR 20180711 1

<h4>Background</h4>Vitamin D has anticarcinogenic and immune-related properties and may protect against some diseases, including breast cancer. Vitamin D affects gene transcription and may influence DNA methylation.<h4>Methods</h4>We studied the relationships between serum vitamin D, DNA methylation, and breast cancer using a case-cohort sample (1070 cases, 1277 in subcohort) of non-Hispanic white women. For our primary analysis, we used robust linear regression to examine the association betwee ...[more]

PMID: 29996894

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Dataset Information

Vitamin D, DNA methylation, and breast cancer.

Background

Methods

Results

Conclusions

Publications

Vitamin D, DNA methylation, and breast cancer.

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