Unknown

Dataset Information

0

AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes.


ABSTRACT: Podocytes are highly-specialized epithelial cells essentially required for the generation and the maintenance of the kidney filtration barrier. This elementary function is directly based on an elaborated cytoskeletal apparatus establishing a complex network of primary and secondary processes. Here, we identify the actin-bundling protein allograft-inflammatory-inhibitor 1 like (AIF1L) as a selectively expressed podocyte protein in vivo. We describe the distinct subcellular localization of AIF1L to actin stress fibers, focal adhesion complexes and the nuclear compartment of podocytes in vitro. Genetic deletion of AIF1L in immortalized human podocytes resulted in an increased formation of filopodial extensions and decreased actomyosin contractility. By the use of SILAC based quantitative proteomics analysis we describe the podocyte specific AIF1L interactome and identify several components of the actomyosin machinery such as MYL9 and UNC45A as potential AIF1L interaction partners. Together, these findings indicate an involvement of AIF1L in the stabilization of podocyte morphology by titrating actomyosin contractility and membrane dynamics.

SUBMITTER: Yasuda-Yamahara M 

PROVIDER: S-EPMC6042786 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

altmetric image

Publications

AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes.

Yasuda-Yamahara Mako M   Rogg Manuel M   Yamahara Kosuke K   Maier Jasmin I JI   Huber Tobias B TB   Schell Christoph C  

PloS one 20180712 7


Podocytes are highly-specialized epithelial cells essentially required for the generation and the maintenance of the kidney filtration barrier. This elementary function is directly based on an elaborated cytoskeletal apparatus establishing a complex network of primary and secondary processes. Here, we identify the actin-bundling protein allograft-inflammatory-inhibitor 1 like (AIF1L) as a selectively expressed podocyte protein in vivo. We describe the distinct subcellular localization of AIF1L t  ...[more]

Similar Datasets

| S-EPMC3688894 | biostudies-literature
| S-EPMC10491668 | biostudies-literature
2010-08-24 | E-GEOD-23764 | biostudies-arrayexpress
| S-EPMC8025214 | biostudies-literature
2010-08-24 | GSE23764 | GEO
| S-EPMC3226764 | biostudies-literature
| S-EPMC3340076 | biostudies-literature
| S-EPMC10112912 | biostudies-literature
| S-EPMC6031329 | biostudies-literature
| S-EPMC3896915 | biostudies-literature