Dataset Information

B7-H4 overexpression contributes to poor prognosis and drug-resistance in triple-negative breast cancer.

ABSTRACT:

Background

The expression of the immunoregulatory protein B7-H4 has been reported in many types of cancer, including breast cancer. However, its role in triple-negative breast cancer (TNBC), especially its correlation with patients' prognosis and chemoresistance remains unclear.

Methods

The expression of B7-H4 in TNBC tissues and cell lines were measured with Real-Time PCR and western blotting. 65 cases of TNBC tissue samples and adjacent non-tumor tissue samples were analyzed by immunochemistry to demonstrate the correlation between the B7-H4 expression and clinicopathological characteristics. In vitro studies assessed mAb MIH43 alone and in combination with transfecting B7-H4 siRNA on the growth of chemosensitive and chemoresistant TNBC cell lines by CCK-8 and apoptotic enzyme-linked immunosorbent assay (ELISA).

Results

B7-H4 expression was detected positive in 59 of 65 (90.8%) different stage TNBC patients, especially in the samples of recurrence TNBC patients after receiving neoadjuvant chemotherapy treatment. Survival curves showed that patients with B7-H4 overexpression had significantly shorter survival and recurrence time than those with low B7-H4 expression (p?ConclusionsOur data show that B7-H4 is a biomarker indicative of a poor prognosis in TNBC patients and at least partially downregulated in chemoresistance via PTEN/PI3K/AKT pathway. Targeting B7-H4 might provide an attractive therapeutic approach specifically for TNBC patients.

SUBMITTER: Wang L

PROVIDER: S-EPMC6044050 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

B7-H4 overexpression contributes to poor prognosis and drug-resistance in triple-negative breast cancer.

Wang Ling L Yang Chao C Liu Xin-Bo XB Wang Li L Kang Fu-Biao FB

Cancer cell international 20180713

<h4>Background</h4>The expression of the immunoregulatory protein B7-H4 has been reported in many types of cancer, including breast cancer. However, its role in triple-negative breast cancer (TNBC), especially its correlation with patients' prognosis and chemoresistance remains unclear.<h4>Methods</h4>The expression of B7-H4 in TNBC tissues and cell lines were measured with Real-Time PCR and western blotting. 65 cases of TNBC tissue samples and adjacent non-tumor tissue samples were analyzed by ...[more]

PMID: 30008617

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Dataset Information

B7-H4 overexpression contributes to poor prognosis and drug-resistance in triple-negative breast cancer.

Background

Methods

Results

Publications

B7-H4 overexpression contributes to poor prognosis and drug-resistance in triple-negative breast cancer.

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