Ontology highlight
ABSTRACT:
SUBMITTER: Ushimaru K
PROVIDER: S-EPMC6044693 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
ACS omega 20170123 1
The initial stage of in vitro polyhydroxyalkanoate (PHA) polymerization by PHA synthase from <i>Ralstonia eutropha</i> (PhaC<sub>Re</sub>) on a mica substrate in water was observed using high-speed scanning atomic force microscopy (HS-AFM). Before PHA polymerization, the adsorption-desorption cycle of the PhaC<sub>Re</sub> molecule on mica was observed in real time. For approximately 30 s after the addition of the PHA monomer, no significant change was observed on the mica substrate, but PhaC<su ...[more]