Dataset Information

Isolation and identification of a bacterial cellulose synthesizing strain from kombucha in different conditions: Gluconacetobacter xylinus ZHCJ618.

ABSTRACT: A bacterial cellulose (BC) synthesizing strain (Gluconacetobacter xylinus ZHCJ618) was isolated from kombucha and selected as the species for commercial applications owing to its high phenotypic stability and sustainable production capacity of 7.56 ± 0.57 g/L under static culturing conditions and 8.31 ± 0.79 g/L under shaking conditions. The morphological, physiological and biochemical characteristics of the strain were similar to those of Gluconacetobacter genus. The 16S rDNA sequence homologies with G. xylinus NCIB 11664 reached 99%, showing that the isolated strain can be identified as G. xylinus. The material properties of BC were studied by fourier transform infrared spectroscopy, scanning electronic microscopy, X-ray diffraction, thermogravimetric analysis, and tensile test. The results showed that BC synthesized under static conditions exhibited stronger tear strength, higher crystallinity, superior waterhold and rehydration rate than BC synthesized under shaking conditions.

SUBMITTER: Zhang W

PROVIDER: S-EPMC6049678 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Isolation and identification of a bacterial cellulose synthesizing strain from kombucha in different conditions: Gluconacetobacter xylinus ZHCJ618.

Zhang Wen W Wang Xuechuan X Qi Xiangjun X Ren Longfang L Qiang Taotao T

Food science and biotechnology 20180111 3

A bacterial cellulose (BC) synthesizing strain (Gluconacetobacter xylinus ZHCJ618) was isolated from kombucha and selected as the species for commercial applications owing to its high phenotypic stability and sustainable production capacity of 7.56 ± 0.57 g/L under static culturing conditions and 8.31 ± 0.79 g/L under shaking conditions. The morphological, physiological and biochemical characteristics of the strain were similar to those of Gluconacetobacter genus. The 16S rDNA sequ ...[more]

PMID: 30263796

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Project description:Gluconacetobacter hansenii, a Gram-negative bacterium, produces and secrets highly crystalline cellulose into growth medium, and has long been used as a model system for studying cellulose synthesis in higher plants. Cellulose synthesis involves the formation of ?-1,4 glucan chains via the polymerization of glucose units by a multi-enzyme cellulose synthase complex (CSC). These glucan chains assemble into ordered structures including crystalline microfibrils. AcsA is the catalytic subunit of the cellulose synthase enzymes in the CSC, and AcsC is required for the secretion of cellulose. However, little is known about other proteins required for the assembly of crystalline cellulose. To address this question, we visually examined cellulose pellicles formed in growth media of 763 individual colonies of G. hansenii generated via Tn5 transposon insertion mutagenesis, and identified 85 that produced cellulose with altered morphologies. X-ray diffraction analysis of these 85 mutants identified two that produced cellulose with significantly lower crystallinity than wild type. The gene disrupted in one of these two mutants encoded a lysine decarboxylase and that in the other encoded an alanine racemase. Solid-state NMR analysis revealed that cellulose produced by these two mutants contained increased amounts of non-crystalline cellulose and monosaccharides associated with non-cellulosic polysaccharides as compared to the wild type. Monosaccharide analysis detected higher percentages of galactose and mannose in cellulose produced by both mutants. Field emission scanning electron microscopy showed that cellulose produced by the mutants was unevenly distributed, with some regions appearing to contain deposition of non-cellulosic polysaccharides; however, the width of the ribbon was comparable to that of normal cellulose. As both lysine decarboxylase and alanine racemase are required for the integrity of peptidoglycan, we propose a model for the role of peptidoglycan in the assembly of crystalline cellulose.

Dataset Information

Isolation and identification of a bacterial cellulose synthesizing strain from kombucha in different conditions: Gluconacetobacter xylinus ZHCJ618.

Publications

Isolation and identification of a bacterial cellulose synthesizing strain from kombucha in different conditions: <i>Gluconacetobacter xylinus</i> ZHCJ618.

Similar Datasets

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