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Nanocapsule-mediated cytosolic siRNA delivery for anti-inflammatory treatment.

ABSTRACT: The use of nanoparticle-stabilized nanocapsules for cytosolic siRNA delivery for immunomodulation in vitro and in vivo is reported. These NPSCs deliver siRNA directly to the cytosol of macrophages in vitro with concomitant knockdown of gene expression. In vivo studies showed directed delivery of NPSCs to the spleen, enabling gene silencing of macrophages, with preliminary studies showing 70% gene knockdown at a siRNA dose of 0.28?mg/kg. Significantly, the delivery of siRNA targeting tumor necrosis factor-? efficiently silenced TNF-? expression in LPS-challenged mice, demonstrating efficacy in modulating immune response in an organ-selective manner. This research highlights the potential of the NPSC platform for targeted immunotherapy and further manipulation of the immune system.

SUBMITTER: Jiang Y

PROVIDER: S-EPMC6054481 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Nanocapsule-mediated cytosolic siRNA delivery for anti-inflammatory treatment.

Jiang Ying Y Hardie Joseph J Liu Yuanchang Y Ray Moumita M Luo Xiang X Das Riddha R Landis Ryan F RF Farkas Michelle E ME Rotello Vincent M VM

Journal of controlled release : official journal of the Controlled Release Society 20180605

The use of nanoparticle-stabilized nanocapsules for cytosolic siRNA delivery for immunomodulation in vitro and in vivo is reported. These NPSCs deliver siRNA directly to the cytosol of macrophages in vitro with concomitant knockdown of gene expression. In vivo studies showed directed delivery of NPSCs to the spleen, enabling gene silencing of macrophages, with preliminary studies showing 70% gene knockdown at a siRNA dose of 0.28 mg/kg. Significantly, the delivery of siRNA targeting tumor necros ...[more]

PMID: 29883695

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