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Chemically controlled aggregation of pluripotent stem cells.

ABSTRACT: Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical-based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension-based manufacturing of PSC cultures that are critical for the translation of regenerative medicine strategies to clinical products.

SUBMITTER: Lipsitz YY

PROVIDER: S-EPMC6055717 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Chemically controlled aggregation of pluripotent stem cells.

Lipsitz Yonatan Y YY Tonge Peter D PD Zandstra Peter W PW

Biotechnology and bioengineering 20180501 8

Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical-based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension-based manufac ...[more]

PMID: 29679475

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