Dataset Information

Enhanced virulence of Fusarium species associated with spear rot of oil palm following recovery from osmotic stress.

ABSTRACT: Fusarium spp., which are common inhabitants of oil palm leaves, are weak pathogens of common spear rot (CSR). We investigated the influence of osmotic stress on the growth, virulence, and activity of cell wall-degrading enzymes of CSR fungi, using potato dextrose agar (PDA) supplemented with KCl or sucrose (hyperosmotic medium). Hyperosmotic stress significantly inhibited mycelial growth, but growth rapidly recovered when mycelia were transferred to control medium. When inoculated into oil palm spear leaflets, Fusarium sp., and F. incarnatum precultured on 1.0 and 1.5 M KCl-hyperosmotic medium induced lesions that were two to four times larger than those in non-stressed cultures, suggesting enhanced virulence of the weak pathogens. Lesion size was not greatly affected in hyperosmotic cultures of moderately virulent F. sacchari. No activity of pectin lyase was detected in liquid cultures of the Fusarium isolates. All isolates except F. incarnatum BT48 secreted polygalacturonase (PG), which was active in both liquid cultures and inoculated leaves. Significantly increased PG activity (5-32-fold) was observed on leaves inoculated with hyperosmotic cultures of Fusarium sp. and F. sacchari. These findings suggest that Fusarium sp., F. incarnatum, and F. sacchari exhibit an adaptive physiological plasticity to hyperosmotic stress that results in enhanced virulence.

SUBMITTER: Suwandi S

PROVIDER: S-EPMC6059076 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Low salinity waterflooding (low salinity-EOR) has attracted great interest from many giant oil producers and is currently under trial in some of the oil fields of the United States, Middle Eastern countries, and North Sea reservoirs. Most of the reported studies on this process were carried out for medium to relatively heavy oil with significant polar contents. In this work, we have investigated low salinity waterflooding performance for light paraffinic crude oil with a low acid number. This study has been performed using crude oil from an Indian offshore oilfield and Indian offshore seawater. Oil recovery efficiencies of seawater and its diluted versions (low salinity seawater) were evaluated through core-flooding experiments performed on a silica sand pack containing small amounts (2 wt %) of bentonite clay saturated with crude oil. Interfacial tension and wettability studies were performed to understand the associated low salinity effects on the crude oil/brine/rock properties. Effluent brine produced during the flooding experiments was also analyzed to obtain a clearer insight into the low salinity-enhanced oil recovery (EOR) mechanism. The results showed that injection of low salinity seawater can significantly increase the waterflood recovery in comparison with high salinity seawater injection. Interfacial tension and contact angle studies revealed that there is an optimum dilution level at which the interfacial tension and wettability are the most favorable for enhanced oil recovery even in the case of light paraffinic crude. These results are in line with the results obtained from the core-flooding experiments. The possible reason behind recovery improvement based on the interfacial tension and wettability studies in conjugation with the effluent brine analysis has been discussed in detail. In this study, we have observed that the enhanced oil recovery efficiency could be achieved by applying low salinity seawater flooding even in the case of light paraffinic oil with a low acid number.

Project description:The detrimental stresses of future climate change are well known and decisions are required to reduce their effects. Climate and disease stresses cause severe damage to plants and it is essential to understand how they will respond. Oil palm (OP) is an Fusarium important crop for many countries. The palm originated in Africa, where palm oil is produced in the largest amount within the continent by Nigeria. OP becomes stressed by climate change and wilt, a devastating disease of OP in Africa. Previous methods to determine the suitability of future climate on OP in continents and whole countries were applied to Nigeria, which is the first time an individual country has been assessed in this manner. Climate maps of Nigeria were divided equally into 16 regions from north to south and east to west to determine the future suitable climate for growing OP. CLIMEX and narrative modelling were used to determine suitability for growing OP and Fusarium wilt incidence for current time and 2050. Maps from published papers were employed directly thereby facilitating the procedure. A distinct latitudinal increasing trend from north to south in suitable climate was observed, which was unexpected. A decreasing longitudinal trend from west to east was also observed. These differences in suitable climates may allow refuges for OP in the future. The growth of OP in the south of Nigeria may be largely unaffected by climate change by 2050, unlike the north. The procedures allow policy decisions at state and national levels to be made from empirical data, which do not otherwise exist. States with low amounts of OP and where the climate deteriorates greatly, could usefully be abandoned. Other low palm oil producers, where the climate does not deteriorate greatly, could be encouraged to develop OP. Little requires to be done in the high producing states where the climate does not deteriorate. In all cases, the environmental impacts require thorough assessment. Climate change requires reduction as indicated in recent Conference of the Parties meetings.

Dataset Information

Enhanced virulence of Fusarium species associated with spear rot of oil palm following recovery from osmotic stress.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets