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Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution.

ABSTRACT: Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here, we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy, this technique allowed us to repeatedly image neurons, microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation. Given its easy handling and safety, this method holds great promise for application in neuroscience research.

SUBMITTER: Zhao YJ

PROVIDER: S-EPMC6060065 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Skull optical clearing window for <i>in vivo</i> imaging of the mouse cortex at synaptic resolution.

Zhao Yan-Jie YJ Yu Ting-Ting TT Zhang Chao C Li Zhao Z Luo Qing-Ming QM Xu Tong-Hui TH Zhu Dan D

Light, science & applications 20180223

Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here, we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy, this technique allowed us to repeatedly image neurons, microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation ...[more]

PMID: 30839532

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