Ontology highlight
ABSTRACT:
SUBMITTER: Yang G
PROVIDER: S-EPMC4690457 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Nature protocols 20100114 2
Imaging neurons, glia and vasculature in the living brain has become an important experimental tool for understanding how the brain works. Here we describe in detail a protocol for imaging cortical structures at high optical resolution through a thinned-skull cranial window in live mice using two-photon laser scanning microscopy (TPLSM). Surgery can be performed within 30-45 min and images can be acquired immediately thereafter. The procedure can be repeated multiple times allowing longitudinal ...[more]